Cell-Surface Vimentin Monoclonal Antibody

Vimentin, a 57kDa type III intermediate filament, is responsible for architecture of cytoplasm.1 As a popular intracellular epithelial mesenchymal transition (EMT) marker, overexpression of vimentin in cancer cells was known for its high correlation with cancer progression. Apart from secretion under some circumstances, vimentin can also be recruited to the cell surface, known as cell surface vimentin (CSV), to participate in cell adhesion, migration and cellular signaling.2 The significance of CSV is related to its involvement in autoimmune disorders, viral infection and cancer. CSV expression on activated marcophages, platelets and apoptotic T lymphocytes evidenced its connection to human neutrophil spontaneous apoptosis further supports its association with inflammatory diseases.3 Being a putative anti-viral drug target, CSV also takes parts in viral multiplication by directly facilitate internalization of virion.4 Recently, breakthroughs on identifying CSV as cancer specific EMT marker, in association with metastatic phenotypes in aggressive cancer and sarcoma, has made an impact on cancer research community. Because unlike intracellular vimentin, the cell surface detection of CSV enables the isolation of cancer cells.5,6,7 Abnova obtains worldwide exclusive license of the patented CSV monoclonal antibody, clone 84-1, from MD Anderson Cancer Center, United States. This antibody has been validated for its intended use in majorities of cancer cell lines by both immunofluoresence and flow cytometry.8 As a sole provider for this powerful tool, Abnova hopes to propel the investigations on cancer research with specificity and broad applicability. CSV monoclonal antibody, clone 84-1 H00007431-M08 CSV Expression in Different Cell Lines8 Cell Line CSV Cell Line CSV Cell Line CSV Breast   Bone   Liver     MCF-7 (H) +   OS-25 (H) ++   AMC14 (M) ++   SKBR3 (H) +   HOS (H) ++ Colon     MDA-MB-231 (H) +   MG-263 (H) ++   DLD-1 (H) ++   MDA-MB-453 (H) +   LM7 (H) +   GEO (H) ++   MDA-MB-458 (H) ++   SAOS-2 (H) +   OS-187 (H) ++   4T1 (M) +   OS25 (H) +   SW620 (H) + Brain     OS-O (H) ++   SW480 (H) +   SKNAS (H) ++   OS-D (H) +   HCT-116 (H) +   SKNBE2 (H) +++   U2OS (H) +   HT-29 (H) ++   NGP (H) +   CCH-OSD (H) +   Caco-2 (H) +   SH-SY5Y (H) ++   K7 (M) ++   CT-26 (M) +   LAN5 (H) ++   K7M2 (M) +++ Pancreas     KCN (H) +   DUNN (M) +   PANC-1 (H) ++   DBT (M) +   LM8 (M) +++   MiaPACA-2 (H) +   U251 (H) +   OST-DL-391 + Other   Bladder     OST-DL-393 +   FBL3 (M) +++   RT4V6 (H) +   OST-DL-396a ++   SCCVII (M) +   T24 (H) ++         Flow Cytometry Using CSV monoclonal antibody, clone 84-1 (H00007431-M08) CSV Expression in Cancer Cell Lines Immunological assessment of CSV in various cancer cell lines and normal cell lines using flow cytometry in which only cancer cells, including GEO (colorectal cancer), MDA-MB-231 (breast cancer) and PANC-1 (pancreatic cancer) can be detected by CSV, 84-1.6 CSV Expression in Sarcoma Cell Lines Immunological assessment of CSV in various cancer cell lines and normal cell lines using flow cytometry in which only cancer cells, including SKNBE-2 (neuroblastoma), RH-41 (rhabdomyosarcoma) and LM7 (osteosarcoma) can be detected by CSV, 84-1.5 Sorting of Hepatocellular Carcinoma Cells (HCC) Stem-cell liked cancer cell populations, csVim+ CD133- and csVim- CD133+, were isolated and sorted into sub-G1, G1, S and G2 phases from primary HCC using CSV, 84-1.7 Immunofluorescence Using CSV monoclonal antibody, clone 84-1 (H00007431-M08) Analysis of CSV-specific 84-1 monoclonal antibody Cell surface staining of CSV in LM7 (osteosarcoma) cancer cell line using confocal microscopy. Cells were stained for CSV (green), WGA (red) and nucleus DRAQ5 (blue). CSV monoclonal antibody 84-1 co-localized with WGA indicated cell surface vimentin.5 Detection of CSV in HPC-1-derived Spheres on Geltrex-coated Wells Vimentin (green) was detected at the periphery of 3-dimensional HPC-derived sphere model. Since cells at periphery were more aggressive, the invasivephenotype correlated with increase nuclear accumulation of β-catenin (red).6 Detection of CSV on Human Colorectal Cancer Cells Circulating tumor cells derived from colorectal cancer patients were stained for nucelus (blue), cell surface vimentin (green) and specific EMT biomarkers (red), including FOXC2, TWIST-2, SNAIL, SLUG, EpCAM and E-cadherin.6 References 1. Mitra, A. and Li, S. (2014). J Cancer Prev Curr Res. DOI: 10.15406/jcpcr.2014.01.00014 2. Satelli, A. and Li, S. (2011). Cell Mol Life Sci. DOI: 10.1007/s00018-011-0735-1 3. Moisan, E. and Girard, D. (2005). JLB. DOI:10.1189/jlb.0405190 4. Yu, YT., et. al. (2016). Journal of Biomedical Science. DOI: 10.1186/s12929-016-0234-7 5. Satelli A., et al. (2014). Cancer Research. DOI:10.1158/0008-5472.CAN-13-1739. 6. Satelli A., et al. (2014). Clinical Cancer Research. DOI:10.1158/1078-0432.CCR-14-0894. 7. Mitra, A., et al. (2015). IJC. DOI:10.1002/ijc.29382. 8. Satelli A. (2014). WIPO. WO 2014/138183 A1. Related Products   Proteoliposome Expression Kit for High Performance Membrane Protein Expression

RAS Signaling Cancer Research

New Discoveries on RAS Signaling Cancer Research ERK inhibitor alone can suppress the growth of KRAS-mutant pancreatic cancer cell lines and in combination with PI3K inhibition, they can cause synergistic cell death. However, long-term treatment of ERK inhibitor has found to create unexpected senescence and resistence mediated through the degradation of MYC and enhanced basal signaling of PI3K-AKT-mTOR, resepctively. (Cancer Cell,2016) Combination treatment of trametinib and palbociclib on KRAS-mutant colorectal cancer patient derived xenograft models was fround well tolerated and efficacious; it has demonstrated the concept of co-targeting MEK and CDK4/6 can result in anti-tumor activity. This approach may improve the therapeutic outcome for those who are suffering from metastatic colorectal cancer in the future. (Clinical Cancer Research,2016) Through the activation and postive feedback of Ras/Raf/MEK/ERK pathway, Hepatitis C virus (HCV) facilitates the hepatoma cell proliferation. Recently, bromodomain containing 7 (BRD7), a tumor suppressor triggered by Ras/Raf/MEK/ERK signaling, was found to attenuate hepatocellular carcinoma (HCC) development through the negative regulation of the same pathway. Thus, the balance between BRD7 and Ras/Raf/MEK/ERK activity is crucial for the regulation of HCV derived HCC . (Cancer Letters,2016) Ras Signaling for Cancer Growth Suppression (Click to see your products of interest) Eight Product Categories and 1500+ Products 303 Monoclonal Antibodies 638 Polyclonal Antibodies 221 Antibody Pairs 135 Recombinant Proteins 67 ELISA and Assay Kits 85 RNAi 28 FISH Probes 33 Cell Transient Overexpression Lysates Featured Products KRAS monoclonal antibody (M01), clone 3B10-2F2 H00003845-M01 Immunofluorescence staining of HeLa cells with KRAS monoclonal antibody (M01), clone 3B10-2F2 at 10 ug/mL. PDK1 monoclonal antibody, clone 4A11 MAB10380 Immunohistochemsitry analysis of paraffin-embedded human breast cancer tissue using PDK1 monoclonal antibody, clone 4A11 with DAB staining. MTOR monoclonal antibody (M01), clone 2C5 H00002475-M01 Proximity Ligation Analysis of protein-protein interactions between AKT1 and MTOR. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal at 1:1200 and anti-MTOR mouse monoclonal antibody at 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). References Hayes, T.K., et.al. (2016). Cancer Cell. DOI:10.1016/j.ccell.2015.11.011 Ziemke, E.K., et.al. (2016). Clinical Cancer Research. DOI:10.1158/1078-0432.CCR-15-0829 Zhang, Q., et. al. (2016). Cancer Letters. DOI:10.1016/j.canlet.2015.11.027 New Products   270+ High standard IHC antibodies and 30 ELISA Kits to enhance your research

Neurodegenerative Disorder

New Discoveries on Neurodegenerative Disorder Strong evidence has emerged to implicate disturbed mitochondrial fusion and fission as central pathological components underpinning a number of neurodegenerative disorders. Imbalances in mitochondrial fusion and fission affects respiratory chain function, mitochondrial quality control (mitophagy), and programmed cell death. The same cellular processes are also implicated in more-common complex neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. This indicates a common pathological thread and a close relationship among mitochondrial structure, function and localization. (Nature Reviews Neurology, 2015) A mitochondrial fission arrest phenotype may cause elongated interconnected organelles, "mitochondria-on-a-string" (MOAS). Findings suggest that MOAS formation may occur at the final stages of fission process and was not associated with altered translocation of activated dynamin related protein 1 (Drp1) to mitochondria but with reduced GTPase activity. The findings on this research brings new attention to the major role of mitochondrial dynamics in regulating neuronal survival. (Scientific Reports, 2016) Mutations in Parkin and PINK1 cause an inherited early-onset form of Parkinson's disease. The two proteins function together in a mitochondrial quality control pathway. Here, a binding switch between phospho-ubiquitin (pUb) and the ubiquitin-like domain (Ubl) of Parkin was found to play a key role in the release of auto-inhibited ubiquitin ligase activity of Parkin. (The EMBO J, 2015) Neurodegenerative Disorders Alzheimer's Disease Abs Huntington's Disease Abs Parkinson's Disease Abs Mitochondria Implicated Neurodegenerative Disorder Pathways (Click to see your products of interest) Eight Product Categories and 1000+ Products 250 Monoclonal Antibodies 397 Polyclonal Antibodies 35 Antibody Pairs 201 Recombinant Proteins 8 ELISA and Assay Kits 189 RNAi 9 FISH Probes 100 Cell Transient Overexpression Lysates Featured Products IGH/BCL2 DY Translocation FISH Probe FT0011 Fluorescent In Situ Hybridization of human cholangiocarcinoma (FFPE) with IGH/BCL2 DY Translocation FISH Probe (Cat # FT0011). IGH (FITC) BCL2 (Texas Red) PARK2 monoclonal antibody (M01), clone 1H4 H00005071-M01 Immunofluorescence of HeLa Cells with PARK2 monoclonal antibody (Cat # H00005071-M01) at 10 ug/mL. BCL2L1 & BAX Protein Protein Interaction Antibody Pair DI0068 Proximity Ligation Assay of HeLa cells with Anti-BCL2L1 rabbit purified polyclonal antibody (1:1200) and anti-BAX mouse monoclonal antibody (1:50). DAPI (blue) References Florence Burté, et.al. (2015). Nature Review Neurology. DOI: 10.1038/nrneurol.2014.228 Liang Zhang, et. al. (2016) Nature Scientific Reports, DOI: 10.1038/srep18725 Véronique Sauvé, et. al. (2015) The EMBO Journal. DOI: 10.15252/embj.201592237 New Products   200 New Monoclonal antibodies for IHC staining to accelerate your work with precision and accuracy

RNAi Regulations

New Discoveries through RNA interference RNA interference has been developed as methods to evaluate IKK-NF-κB pathway in lung cancer through the influence of IKKα or IKKβ inhibition on NF-κB transcriptional activity and the effects of siRNA-mediated IKK inhibition on cancer proliferation. (Methods in Molecular Biology,2015) High-throughput RNAi kinome screen targeting 720 kinases has identified PDK1 as a factor mediating resistance to CDK4/6 inhibition; thus, it can be a potential drug target in combination with CDK4/6 inhibitor LEE011 to treat ER+ breast cancer. (Cancer Research,2015) Through RNA interference screen, genes, such as TACR1, were identified as factors involved in stress response of mammalian cells and further provides new approaches to reduce the mortality caused by renal ischemia. (Cell Death & Differentiation,2015) RNAi Pathways (Click to see your products of interest) 16 Product Categories 15000+ Products   Antibody Validated Chimera Pre-design Chimera Angiogenesis 2 40 Apoptosis 14 470 Binding 65 2671 Cell Ad/ Junc/ Cytoskeleton 10 535 Cell Cycle 30 704 Cytokine N/A 56 Enzyme 52 793 Membrane 40 2778 Metabolism 13 333 Neurobiology 3 151 Plasma/ Serum 25 860 Signal Transduction 13 623 Stem Cell 10 239 Transcriptions 45 957 Ubiquitin 6 441 Others 45 3200 Featured Products EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 H00027161-M01 Immunohistochemistry staining of formalin fixed paraffin embedded human stomach with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 at 3 ug/mL. NBR1 Validated Chimera RNAi H00004077-R01V Western blot analysis of NBR1 over-expressed 293 cell line, cotransfected with NBR1 Validated Chimera RNAi. Lane 1. Non-transfected control Lane 2. Transfected with NBR1 validated chimera RNAi Blot was probed with NBR1 monoclonal antibody (M05), clone 5C3 (Cat # H00004077-M05 ). GAPDH was used as loading control. TARDBP Validated Chimera RNAi H00023435-R01V Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi. Lane 1. Non-transfected control Lane 2. Transfected with TARDBP validated chimera RNAi Blot was probed with TARDBP monoclonal antibody (M01), clone 2E2-D3 (Cat # H00023435-M01 ). GAPDH was used as loading control. References Bassères, D.S., et.al. (2015). Methods in Molecular Biology. DOI:10.1007/978-1-4939-2422-6_27 Jansen, V.M., et.al. (2015). Cancer Research. DOI:10.1158/1538-7445.AM2015-2844 Zynda, E.R.., et. al. (2015). Cell Death & Differentiation. DOI:10.1038/cdd.2015.128 New Products   High standard IHC antibodies, Rabbit monoclonal antibodies and FISH Probes to fullfill your needs

Epigenetic Modifications

New Discoveries on Epigenetics H1 functions intrinsically to promote germline stem cells (GSC) self-renewal by antagonizing males absent on the first (MOF) function. The findings of H1 and H4K16 acetylation function might be applicable to stem cells in other species since H1 and H4K16 acetylation are highly conserved from fly to human. (Nature Communication, 2015) Overexpression of a related H3K9me3 demethylase KDM4A improves human somatic cell nuclear transfer (SCNT). As H3K9me3 is a SCNT reprogramming barrier in human somatic cell genome, the overexpression of KDM4A facilitates transcriptional reprogramming and therefore, improves SCNT. This conserved mechanistic insight has potential applications for improving SCNT in a variety of contexts, including regenerative medicine. (Cell Stem Cell, 2015) H4 tetra-acetylation is found to impair Nucleosome core particles (NCP) self-association by changing the interactions of the H4 tail with DNA, and is the first demonstration of crystallization quality NCPs reconstituted with genuine post-translational modifications (PTMs). (Nature Scientific Reports, 2015) Epigenetic Regulation (Click to see your products of interest) Epigenetic Gene Regulation: Modification Methylation Acetylation Mono-methylation Di-methylation Tri-methylation DNA Repression -- -- -- Histone H3K4 Activation Activation Activation -- H3K9 Activation Repression Repression Activation H3K27 Activation Repression Repression -- H3K36 -- Repair Activation Activation H3K79 Activation Activation Activation Repression -- H3R17 -- Activation -- -- H4K5 -- -- -- Activation H4K8 -- -- -- Activation H4K12 -- -- -- Activation H4K16 -- -- -- Activation H4K20 Activation Activation Repression -- H4K16 -- -- -- Activation Eight Product Categories and 1000+ Products 197 Monoclonal Antibodies 361 Polyclonal Antibodies 45 Antibody Pairs 108 Recombinant Proteins 142 ELISA and Assay Kits 148 RNAi 4 FISH Probes 46 Cell Transient Overexpression Lysates References Sun J, et.al. (2015). Nature Communication. DOI: 10.1038/ncomms9856 Mohrin M, et. al. (2015) Cell Stem Cell. DOI: 10.1016/j.stem.2015.10.001 Wakamori M, et. al. (2015) Nature Scientific Reports, DOI: 1038/srep17204 Featured Products H3K9ac polyclonal antibody (Cat # PAB14098) ELISA test was performed by using serial diluted H3K9ac polyclonal antibody (Cat # PAB14098) against peptide containing histone modification of interest. Dot Blot analysis was performed with 1:2,000 diluted H3K9ac polyclonal antibody (Cat # PAB14098) against peptides with or without histone modifications. Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740) Fluorescent Immunohistochemistry of NIH/3T3 cells was performed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740) at 5 ug/mL. Visualization was done using Cy3 (red) labeled Goat anti Rabbit IgG and DAPI (blue). New Products   300+ Specific monoclonal antibodies for IHC staining to accelerate your work with precision

TGFβ Signaling Pathway

New Discoveries on TGFβ Signaling Pathway ITGBL1, as an upstream effector of TGFβ signaling pathway, is a key contributor to osteomimetic breast cancer. (Cancer Research,2015) Through the activation of TGFβ signaling pathway, HOXB7 promotes malignant tumor progression in cell-autonomous and non-cell-autonomous manners. (Cancer Research,2015) Colon carcinogenesis is strongly associated with genetic mutations in TGFβ signaling pathway of inflamed and regenerating intestinal mucosa. (Cancer Research,2015) TGFβ / SMAD Signaling Pathway (Click to see your products of interest)

Eight Product Categories and 900+ Products
221 Monoclonal Antibodies 301 Polyclonal Antibodies 141 Antibody Pairs 111 Recombinant Proteins	20 ELISA and Assay Kits 76 RNAi 10 FISH Probes 22 Cell Transient Overexpression Lysates

Featured Products
MAP2K4 purified MaxPab mouse polyclonal antibody (B01P) H00006416-B01P
	Immunofluorescent staining of HeLa cell using MAP2K4 purified MaxPab mouse polyclonal antibody (Cat# H00006416-B01P) at 10 ug/mL.
LIMK1 monoclonal antibody (M01), clone 1A8 H00003984-M01
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human stomach with anti-LIMK1 monoclonal antibody, clone 1A8 (Cat# H00003984-M01) at 3 ug/mL.
SHC1 monoclonal antibody (M01), clone 3F4 H00006464-M01
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human stomach with anti-SHC1 monoclonal antibody, clone 3F4 (Cat# H00006464-M01) at 1 ug/mL.

References

Li, X.-Q., et.al. (2015). Cancer Research. DOI:10.1158/0008-5472.CAN-15-0240 Liu, S., et.al. (2015). Cancer Research. 10.1158/0008-5472.CAN-14-3100 Oshima H., et. al. (2015). Cancer Research. DOI:10.1158/0008-5472.CAN-14-2036

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300+ High quality antibodies and kits designed for Enzyme-Linked ImmunoSorbent Assay (ELISA)