New Discoveries on Hematopoiesis
- Hematopoietic stem cell (HSC) is generated from the hemogenic endothelium (HE), where HE and arterial vascular endothelium (VE) was found to represent separate lineages, on the basis of differential expression of CD73 and CD184. (Nature Cell Biology, 2015)
- HSC regenerative capacity was increased through the inactivation of a histone deacetylase, SIRT7. The interplay of SIRT7 and NRF1 deregulates an unfolded protein response (UPRmt)-mediated metabolic checkpoint which was defined as a reversible contributing factor for HSC aging. (Science, 2015)
- TET1, the founding member of TET family, was reported as an epigenetic guardian for lymphomagenesis and the TET1 deficiency contributed to predisposed development of B cell malignancy. (Nature Immunology, 2015)
Blood Cell Specific Markers
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| Cell Type |
Markers (Human) |
Markers (Mouse) |
| Hematopoietic Stem Cells |
Lin-, CD34+, CD38-, FLT3+, THY1+ |
Lin-, Slamf1 (Cd150)+, Kit+, Casp3 (Sca-1)+ |
| Lin- |
MS4A1 (CD20), GYPA (CD235a), CD3, NCAM1 (CD56) |
Ptprc (Cd45ra), Itgam (Cd11b), Cd2, Cd3, Cd4, Cd5, Cd8, Ly6g, Klrb1c, Ly76 |
| Common Lymphoid Progenitor (CLP) |
MME (CD10)+, CD34+, IL7R (CD127)+ |
Cd34+, Il7r (Cd127)+ |
| Common Myelold Progenitor (CMP) |
CD34+, IL3RA (CD123)+, FLT3+ |
Cd34+, Il3ra (Cd123)+, Casp3 (Sca-1)- |
| Granulocyte/Macrophage Progenitor (GMP) |
CD34+, CD38+, IL3RA (CD123)+ |
Cd34+, Cd38+, Il3ra (Cd123)+ |
| Megakaryocyte/Erythroid Progenitor (MEP) |
CD38+, PTPRC (CD45RA)- |
Cd38+, Ptprc (Cd45ra)- |
| Megakaryocytes |
ITGA2B (CD41)+, MT2A (CD42)+, MPL (TPOR)+ |
Itga2b (Cd41)+, Mt2a (Cd42)+, Mpl (Tpor)+ |
| Monocytes |
CD14+, CXCR1+, ITGAM (CD11b)+, CD33 (Siglec-3)+ |
Cd14+, Cxcr1+, Itgam (Cd11b)+, Cd33 (Siglec-3)+ |
| Lymphocytes |
LY9+, SLAMF7+ |
Ly9+, Slamf7+ |
| Natural Killer Cells (NK Cells) |
NCAM1 (CD56)+, CD16+ |
Ncr1 (Cd335) +, Fcgr3 (CD16)+ |
| B Cells |
CD19+, MS4A1 (CD20)+ |
Ptprc (Cd45ra)+, Cd19+, Cd22+ |
| T Cells |
CD2+, CD3+, CD4+, CD8+ |
Cd2+, Cd3+, Cd4+, Cd8+ |
| Granulocytes |
CCR3+, ITGAM (CD11b)+, ANPEP (CD13)+, CD16+, ITGB2 (CD18)+ |
Ccr3+, Itgam (Cd11b)+, Anpep (Cd13)+, Fcgr3 (Cd16)+, Itgb2 (Cd18)+ |
| Basophils |
ANPEP (CD13)+, CD164+, IL3RA (CD123)+, LAMP1 (CD107a)+ |
Anpep+, Cd164+, Il3ra (CD123)+, Lamp1 (Cd107a)+ |
| Neutrophils |
CD66+, SELL (CD62L)+, MPO+ |
Ceacam1 (Cd66)+ , Sell+, Mpo+ |
| Eosinophils |
CD44+, CD69+, EMR1+ |
Cd44+, Cd69+, Emr1+ |
| Erythrocytes |
GYPA (CD235a)+, CD59+ |
Gypa (Cd235a)+, Cd59a+ |
| Dendritic Cells |
IL3RA (CD123)+, CD80+, CD83+, CD86+, HLA-DRA+ |
Il3ra (Cd123)+, Cd80+, Cd83+, Cd86+ |
| Macrophages |
CD14+, CD163+, CD36+, CD68+ |
Cd14+, Cd163+, Cd36+, Cd68+ |
| Platelets |
ITGA2B (CD41)+, ITGB3 (CD61)+, PEAR1+ |
Itga2b (Cd41)+, Itgb3 (Cd61)+, Pear1+ |
Hematopoiesis
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IL6 polyclonal antibody
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Immunofluorescence analysis of GC-7901 cells using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) (Green).
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| References |
Ditadi A, et.al. (2015). Nature Cell Biology. DOI: 10.1038/ncb3161.
Mohrin M, et. al. (2015) Science. DOI: 10.1126/science.1258849.
Rasmussen KD, et. al. (2015) Nature Immunology. DOI: 10.1038/ni.3176 |
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