New Discoveries on Epigenetics
- H1 functions intrinsically to promote germline stem cells (GSC) self-renewal by antagonizing males absent on the first (MOF) function. The findings of H1 and H4K16 acetylation function might be applicable to stem cells in other species since H1 and H4K16 acetylation are highly conserved from fly to human. (Nature Communication, 2015)
- Overexpression of a related H3K9me3 demethylase KDM4A improves human somatic cell nuclear transfer (SCNT). As H3K9me3 is a SCNT reprogramming barrier in human somatic cell genome, the overexpression of KDM4A facilitates transcriptional reprogramming and therefore, improves SCNT. This conserved mechanistic insight has potential applications for improving SCNT in a variety of contexts, including regenerative medicine. (Cell Stem Cell, 2015)
- H4 tetra-acetylation is found to impair Nucleosome core particles (NCP) self-association by changing the interactions of the H4 tail with DNA, and is the first demonstration of crystallization quality NCPs reconstituted with genuine post-translational modifications (PTMs). (Nature Scientific Reports, 2015)
Epigenetic Regulation
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Epigenetic Gene Regulation:
| Modification |
Methylation |
Acetylation |
| Mono-methylation |
Di-methylation |
Tri-methylation |
| DNA |
Repression |
-- |
-- |
-- |
| Histone |
H3K4 |
Activation |
Activation |
Activation |
-- |
| H3K9 |
Activation |
Repression |
Repression |
|
| H3K27 |
|
Repression |
Repression |
-- |
| H3K36 |
-- |
Repair |
|
|
| H3K79 |
|
|
|
-- |
| H3R17 |
-- |
Activation |
-- |
-- |
| H4K5 |
-- |
-- |
-- |
Activation |
| H4K8 |
-- |
-- |
-- |
Activation |
| H4K12 |
-- |
-- |
-- |
Activation |
| H4K16 |
-- |
-- |
-- |
Activation |
| H4K20 |
Activation |
Activation |
Repression |
-- |
| H4K16 |
-- |
-- |
-- |
Activation |
| References |
Sun J, et.al. (2015). Nature Communication. DOI: 10.1038/ncomms9856
Mohrin M, et. al. (2015) Cell Stem Cell. DOI: 10.1016/j.stem.2015.10.001
Wakamori M, et. al. (2015) Nature Scientific Reports, DOI: 1038/srep17204 |
| Featured Products |
| H3K9ac polyclonal antibody (Cat # PAB14098) |
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ELISA test was performed by using serial diluted H3K9ac polyclonal antibody (Cat # PAB14098) against peptide containing histone modification of interest. |
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Dot Blot analysis was performed with 1:2,000 diluted H3K9ac polyclonal antibody (Cat # PAB14098) against peptides with or without histone modifications. |
Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740) |
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Fluorescent Immunohistochemistry of NIH/3T3 cells was performed with Trimethyl-Histone H3 polyclonal antibody (Cat # PAB12740) at 5 ug/mL. Visualization was done using Cy3 (red) labeled Goat anti Rabbit IgG and DAPI (blue). |
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