RAS Signaling Cancer Research

New Discoveries on RAS Signaling Cancer Research ERK inhibitor alone can suppress the growth of KRAS-mutant pancreatic cancer cell lines and in combination with PI3K inhibition, they can cause synergistic cell death. However, long-term treatment of ERK inhibitor has found to create unexpected senescence and resistence mediated through the degradation of MYC and enhanced basal signaling of PI3K-AKT-mTOR, resepctively. (Cancer Cell,2016) Combination treatment of trametinib and palbociclib on KRAS-mutant colorectal cancer patient derived xenograft models was fround well tolerated and efficacious; it has demonstrated the concept of co-targeting MEK and CDK4/6 can result in anti-tumor activity. This approach may improve the therapeutic outcome for those who are suffering from metastatic colorectal cancer in the future. (Clinical Cancer Research,2016) Through the activation and postive feedback of Ras/Raf/MEK/ERK pathway, Hepatitis C virus (HCV) facilitates the hepatoma cell proliferation. Recently, bromodomain containing 7 (BRD7), a tumor suppressor triggered by Ras/Raf/MEK/ERK signaling, was found to attenuate hepatocellular carcinoma (HCC) development through the negative regulation of the same pathway. Thus, the balance between BRD7 and Ras/Raf/MEK/ERK activity is crucial for the regulation of HCV derived HCC . (Cancer Letters,2016) Ras Signaling for Cancer Growth Suppression (Click to see your products of interest) Eight Product Categories and 1500+ Products 303 Monoclonal Antibodies 638 Polyclonal Antibodies 221 Antibody Pairs 135 Recombinant Proteins 67 ELISA and Assay Kits 85 RNAi 28 FISH Probes 33 Cell Transient Overexpression Lysates Featured Products KRAS monoclonal antibody (M01), clone 3B10-2F2 H00003845-M01 Immunofluorescence staining of HeLa cells with KRAS monoclonal antibody (M01), clone 3B10-2F2 at 10 ug/mL. PDK1 monoclonal antibody, clone 4A11 MAB10380 Immunohistochemsitry analysis of paraffin-embedded human breast cancer tissue using PDK1 monoclonal antibody, clone 4A11 with DAB staining. MTOR monoclonal antibody (M01), clone 2C5 H00002475-M01 Proximity Ligation Analysis of protein-protein interactions between AKT1 and MTOR. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal at 1:1200 and anti-MTOR mouse monoclonal antibody at 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). References Hayes, T.K., et.al. (2016). Cancer Cell. DOI:10.1016/j.ccell.2015.11.011 Ziemke, E.K., et.al. (2016). Clinical Cancer Research. DOI:10.1158/1078-0432.CCR-15-0829 Zhang, Q., et. al. (2016). Cancer Letters. DOI:10.1016/j.canlet.2015.11.027 New Products   270+ High standard IHC antibodies and 30 ELISA Kits to enhance your research

TGFβ Signaling Pathway

New Discoveries on TGFβ Signaling Pathway ITGBL1, as an upstream effector of TGFβ signaling pathway, is a key contributor to osteomimetic breast cancer. (Cancer Research,2015) Through the activation of TGFβ signaling pathway, HOXB7 promotes malignant tumor progression in cell-autonomous and non-cell-autonomous manners. (Cancer Research,2015) Colon carcinogenesis is strongly associated with genetic mutations in TGFβ signaling pathway of inflamed and regenerating intestinal mucosa. (Cancer Research,2015) TGFβ / SMAD Signaling Pathway (Click to see your products of interest)

Eight Product Categories and 900+ Products
221 Monoclonal Antibodies 301 Polyclonal Antibodies 141 Antibody Pairs 111 Recombinant Proteins	20 ELISA and Assay Kits 76 RNAi 10 FISH Probes 22 Cell Transient Overexpression Lysates

Featured Products
MAP2K4 purified MaxPab mouse polyclonal antibody (B01P) H00006416-B01P
	Immunofluorescent staining of HeLa cell using MAP2K4 purified MaxPab mouse polyclonal antibody (Cat# H00006416-B01P) at 10 ug/mL.
LIMK1 monoclonal antibody (M01), clone 1A8 H00003984-M01
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human stomach with anti-LIMK1 monoclonal antibody, clone 1A8 (Cat# H00003984-M01) at 3 ug/mL.
SHC1 monoclonal antibody (M01), clone 3F4 H00006464-M01
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human stomach with anti-SHC1 monoclonal antibody, clone 3F4 (Cat# H00006464-M01) at 1 ug/mL.

References

Li, X.-Q., et.al. (2015). Cancer Research. DOI:10.1158/0008-5472.CAN-15-0240 Liu, S., et.al. (2015). Cancer Research. 10.1158/0008-5472.CAN-14-3100 Oshima H., et. al. (2015). Cancer Research. DOI:10.1158/0008-5472.CAN-14-2036

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300+ High quality antibodies and kits designed for Enzyme-Linked ImmunoSorbent Assay (ELISA)

Bioactive Kinases

New Discoveries on Bioactive Kinases MET receptor tyrosine kinase is regulated by suppresor of cytokine signaling 1 (SOCS1) to attenuate oncogenic MET signaling in hepatocellular carcinoma (HCC) and other cancers. MET inhibitors may be useful in treating HCC patients. (Oncogen,2015) EGFR tyrosine kinase inhibitors triggers innate drug resistance through the inhibition of Akt activity and inactivation of Ets-1 to enhance NSCLC cell survival . (PNAS,2015) Phosphorylation of Ser293 on α-subunit of pyruvate dehydrogenase complex by PDK3 causes meiotic spindle morphology disruption, chromosome dis-alignment and ATP level reduction in oocyte maturation. (Molecular and Cellular Endocrinology,2015) Ten Kinase Categories and 394 Products (Click to see your products of interest) 42 Serine / Threonine Kinase: AGC Group 10 Serine / Threonine Kinase: CK1 Group 38 Serine / Threonine Kinase: STE Group 24 Serine / Threonine Kinase: Other Group 145 Tyrosine Protein Kinase 54 Serine / Threonine Kinase: CAMK Group 26 Serine / Threonine Kinase: CMGC Group 22 Serine / Threonine Kinase: TKL Group 6 Serine / Threonine Kinase: Atypical Group 27 Other Kinase Crosstalk Between Kinases and Apoptotic Proteases in Cancer (Click to see your products of interest) This figure has been adapted and altered from López-Otín, C. & Hunter, T., (Nature Revews Cancer,2015). Featured Products BMPR2 (Human) Recombinant Protein P5499 The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein. After kinase reaction was stopped by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: ALK4 inactive mutant. ATP: 100 uM. IRAK4 (Human) Recombinant Protein P5566 The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: IRAK1 peptide. ATP: 1000 uM RET (M918T) (Human) Recombinant Protein P5631 The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CSK peptide. ATP: 100 uM. References Gui, Y., et. al. (2015). Oncogene. DOI:10.1038/onc.2015.20 Phuchareon, J., et.al. (2015). PNAS. DOI:10.1073/pnas.1510733112 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 López-Otín, C. & Hunter, T. (2015). Nature Reveiws Cancer. DOI:10.1038/nrc2823 New Products   200+ High quality antibodies for immunohistochemistry, immunofluorescence or flow cytometry

載脂蛋白研究

載脂蛋白擁有親水與疏水的雙重特性，可與磷脂質和負責運輸的疏水性脂質一起構成脂蛋白粒子後，經由淋巴和血液循環到達目標組織或器官，以達成能量供應、生物分子合成或降解之目的。紅斑性狼瘡、心肌梗塞、阿茲海默症及糖尿病等多種疾病已被發現與載脂蛋白的失衡有關。由於載脂蛋白可作為不同疾病的風險指標，因此像ELISA此類技術便成為定量載脂蛋白的重要診斷工具。亞諾法在此驕傲地為您呈現高達420種以上可辨識人類載脂蛋白的產品，幫您的研究引領成功！ 歡迎點此查看完整「產品清單」 與各類型載脂蛋白有關之疾病 <歡迎點擊以下圖中不同類型的載脂蛋白，可帶您導向相對應的試劑產品頁面供您選購!> 精選最新有文獻引用紀錄的載脂蛋白抗體: APOM 單株抗體 (M03), clone 1G9 (H00055937-M03) 載脂蛋白M (apoM)參予膽固醇代謝與鞘氨醇1-磷酸鹽(Sphingosine-1-phosphate (Sphingosine-1-phosphate, S1P) 的運輸。S1P與發炎性疾病的高度相關性反映出定量apoM已成為未來疾病標記的重要性。在發展高敏感性、高專一性、與可重現性的三明治ELISA上，亞諾法的APOM 單株抗體已被應用為初級檢測抗體，以用於測量細胞質內的apoM。(1) 帶有GST標記之重組APOM蛋白的三明治ELISA結果。用H00055937-M03當抓取抗體時的偵測限制為0.1 ng/ml。 利用西方墨點法偵測帶有GST標記的APOM全長重組蛋白(44KDa)。 請點擊這裡查看更多已有文獻引用紀錄的載脂蛋白抗體… 1. Bosteen MH, Dahlbäck B, Nielsen LB, et.al. J Lipid Res. 2015; pii: jlr.D055947. [Epub ahead of print] 最新ELISA Kits和FISH Probes   ★ 30個ELISA Kits: 用於非人類蛋白的定量測定     ★ 26個雙色FISH Probes: 用於辨識基因分離、擴增或易位