Cytoskeleton

New Discoveries on Cytoskeletons Self-organization of actin cytoskeleton, the dynamic of radial fibers-transverse fibers system, takes crucial role in developing the left-right asymmetry in tissues and embryos. (Nature Cell Biology,2015) Apical and basolateral spectrin cytoskeletons, potential mechano-sensors, regulate Hippo signaling pathway in response to human epithelial cell density. (EMBO,2015) Cytoskeleton-associated proteins, CRMP2/DPYSL2, TPM2, TCP1ε and 14.3.3γ, can be potential biomarkers for small-intestinal neuroendocrine tumors invasion. (Molecular and Cellular Endocrinology,2015) Cytoskeleton Compositions (Click to see your products of interest) Proteins Involved in Cytoskeletons (Click to see your products of interest) Actin Polymerization Protein Function/Activity Cofilin Dissociation from (-) end Severin Severing, capping (+) end, control of length of actin filaments; essential for branch formation Gelsolin Severing, capping (+) end, control of length of actin filaments; essential for branch formation CapZ capping protein Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction Tropomodulin Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction ARPC 2/3 Capping (-) end, side binding and nucleation Classes of Intermediate Filaments Protein Function/Activity I. Acidic cytokeratins Mechanical strength II. Basic cytokeratins Mechanical strength III. Vimentin III. Desmin III. GFAP Maintenance of cell shape Structural support for contractile machinery Maintenance of cell shape IV. Neurofilament (NEFL,NEFM,NEFH) Axon strength; Determines axon size V. Nuclear Lamins (Lamin A, Lamin B) Form nuclear scaffold and shape the nucleus VI. Nestin Unknown Modulation of Microtubule Dynamics Protein Function MAP1 Assembles and stabilizes microtubules MAP2 Assembles and cross-links microtubules to one another and intermediate filaments MAP4 Stabilizes microtubules MAPT Assembles and stabilizes and cross-links microtubules RSN/CLIP1 Cross-links microtubules to chromosomes and endosomes Katanin Microtubule severing STMN1/OP18 Binds to tubulin dimers Eight Product Categories and 2100+ Products 654 Monoclonal Antibodies 655 Polyclonal Antibodies 106 Antibody Pairs 310 Recombinant Proteins 34 ELISA Kits 234 RNAi 15 FISH Probe 135 Cell Transient Overexpression Lysates Featured Products TUBA1A monoclonal antibody (M06), clone 2D2 H00007846-M06 Immunofluorescent staining of HeLa cell using TUBA1A monoclonal antibody (Cat #H00007846-M06) at 10ug/ml. ACTA monoclonal antibody (M02), 4A8-2H3 H00000059-M02 Detection limit for recombinant GST tagged ACTA2 using ACTA2 monoclonal antibody (Cat # H00000059-M02) at approximately 1ng/mL as capture antibody. CTNNA1 polyclonal antibody PAB7024 Immunohistochemistry staining of paraffin embedded human prostate using CTNNA1 polyclonal antibody (Cat# PAB7024) at 3.8μg/mL. References Tee, Y.H., et. al (2015). Nature Cell Biology. DOI: 10.1038/ncb3137 Fletcher, G.C., et.al.(2015). EMBO Journal. DOI 10.15252/embj.201489642 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 New Products   42 High quality Metabolism Assay Kits for the detection and quantification of target proteins 15 Cancer research related new products

Kiss of Death - Ubiquitylation

New Discoveries on Ubiquitylation Elongation of ubiquitin chain, regardless of the linkage, can form polyubiquitin fibrils to initiate autophagy pathway. Incapacity of autophagy can cause cytotoxic accumulation of ubiquitin-positive aggregates leading to neurodegenerative diseases. (Nature communication,2015) Highly expressed in human breast and lung cancers, a new deubiquitinating enzyme, USP36, was identified to regulate c-Myc oncoprotein stability in nucleolus. (PNAS,2015) Itch, an E3 HECT ubiquitin ligase, inhibits MAPK p38α activation through ubiquitylation can be exploited therapeutically to prevent chronic skin inflammation. (Science,2015) Tumor Related Misregulated Proteins in Ubiquitin Modification (Click to see your products of interest) Tumor Type Deregulated Protein Substrate Modification Non-Small-Cell Lung Cancer MDM2 (39) p53/TP53 (151) Polyubiquitylation HAUSP/USP7 (8) MDM2(39) p53/TP53 (151) De-ubiquitylation Renal Cell Carcinoma VHL (16) HIF/HIF1/HIF1α (23) De-ubiquitylation Colorectal Cancer APC (69) Cyclin B (31) Securin Polyubiquitylation Lymphoma KITLG/SCF (25) CDKN1B/p27 (51) Polyubiquitylation Fanconi Anaemia-Related Cancer ATAD3A/FANCL (9) FANCD2 (9) Monoubiquitylation MALT Lymphomas IAP2 BCL10 (15) Polyubiquitylation Lymphoma, AML, Gastric Carcinoma CBL (33) RTKs Multiple Monoubiquitylation Cervical Cancer UBE3A/E6-AP (11) p53/TP53 (151) Polyubiquitylation Breast Cancer BRCA1 (75) Unknown Unknown CBL (33) EGFR (117) De-ubiquitylation Ovarian Cancer F-box protein Cyclin E/CCNE1 (43) De-ubiquitylation ( ) Number of Products Provided by Abnova Tumor Related Misregulated Proteins in Ubiquitin Modification (Click to see your products of interest) Eight Product Categories and 1485 Products 296 Monoclonal Antibodies 529 Polyclonal Antibodies 153 Antibody Pairs 183 Recombinant Proteins 44 Assay Kits and ELISA Kits 180 RNAi 17 FISH Probes 80 Cell Transient Overexpression Lysates Newly Cited Products FBXW7 monoclonal antibody (M02), clone 3D1 H00055294-M02 Yumimoto, K., et al. determined expression of FBXW7, the F-box protein of SCF ubiquitin ligase, can suppress cancer metastasis in either non-cell-autonomous or cell-autonomous manner. Abnova’s FBXW7 monoclonal antibody was used in this study to perform immunocytochemical staining on breast tissues. (JCI, 2015) Immunohistochemistry staining of formalin-fixed paraffin-embedded human lung using FBXW7 monoclonal antibody (3ug/ml). Western Blot detection against FBXW7 partial recombinant protein with GST tag (37.73KDa). CCNE1/CEN19p FISH Probe FG0013 Abnova’s FG0013 CCNE1/CEN19p FISH Probe was recently used by Noske, A., et.al. to evaluate CCNE 1 copy number in the investigation of Cyclin E and URI gene amplification as well as protein expression in different epithelial ovarian cancer subtypes. (Experiment and Molecular Pathology, 2015) Normal human lymphocyte stained with the CCNE1/CEN19p dual color FISH probe at interphase nucleus. Human ovary cancer (FFPE) stained with CCNE1/CEN19p FISH Probe. Human ovary cancer showed no CCNE1 gene amplification. References Morimoto, D., et.al. (2015). Nature communications. DOI: 10.1038/ncomms7116 Sun, X.-X., et.al. (2015). PNAS. DOI: 10.1073/pnas.1411713112 Theivanthiran, B., et.al. (2015). Science. DOI: 10.1126/scisignal.2005903 Yumimoto, K., et.al. (2015) JCI. DOI: 10.172/JCI78782 Noske, A., et.al. (2015). Experiment and Molecular Pathology. DOI: 10.1016/j.yexmp.2014.12.004 New Products   3 FISH Probes for Detecting Gene Amplification in Cancer Cells 49 Pathology Antibodies for High Quality Immunohistochemistry Staining 102 Antibody Pairs for ELISA - Sensitive Detection of Target Proteins

Invasion of Influenza A

New Discoveries on Influenza A Viruses (IAVs) Facilitated by high mutation rate of the viral genome and polymerase, IAVs can cause annual epidemics of approximately 500,000 deaths per year and the function interplay between hemagglutinin and neuraminidase glycoprotein characterizes the pathogenic profile of each viral subtype. (Lancet Infect Dis, 2014) IAVs, mimicked as misfolded protein aggregates, carry unanchored ubiquitin chains to exploit host cell’s aggresome processing machinery for efficient IAV entry and capsid dissociation. (Science, 2014) NiPT5, as a potent anti-IAV agent, can suppress the transcription, translation of vRNA and the synthesis of nucleoprotein to impede the early phase of viral replication. (Scientific Reports, 2015) Influenza A Life Cycle (Click to see your products of interest) Eight Product Categories and 550 Products 152 Monoclonal Antibodies 181 Polyclonal Antibodies 10 Antibody Pairs 5 ELISA Kits 48 RNAi 2 FISH Probes 126 Recombinant Proteins 31 Cell Transient Overexpression Lysates Featured Antibodies Seasonal H1N1 Hemagglutinin polyclonal antibody (Cat # PAB16771) Seasonal Hemagglutinin antibody at 2 ug/mL specifically recognizes seasonal influenza virus A H1N1 but not swine-origin influenza virus (S-OIV) A H1N1 Hemagglutinin protein. Influenza A virus Hemagglutinin H5 monoclonal antibody, clone 4H1C10 (Cat # MAB2763) Influenza A virus Hemagglutinin H5 monoclonal antibody, clone 4H1C10 at 2 ug/mL specifically recognizes Avian H5 but not Season H1 Hemagglutinin protein. Seasonal H1N1 Hemagglutinin polyclonal antibody (Cat # PAB16767) Western blot analysis of Hemaggutinin using recombinant seasonal Hemagglutinin (Lanes A & B) and swine-origin Hemagglutinin (Lanes C & D) with Seasonal H1N1 Hemagglutinin polyclonal antibody (Cat # PAB16767) at 2 ug/mL (Lanes A & C) and Swine H1N1 Hemagglutinin polyclonal antibody (Cat # PAB16769) at 2 ug/mL (Lanes B & D). Newly Cited Antibody Influenza A virus nucleoprotein monoclonal antibody, clone InA108 (MAB1272) Belardo, G., et al. investigated the anti-influenza activity by using combination therapy of NTZ with neuraminidase inhibitors against broad range of Influenza A viruses and found positive outcome showing synergistic effect. Abnova’s anti-nucleoprotein monoclonal antibody was used in this study for the detection of viral replication via immunocytochemical staining. (Antimicrob. Agents Chemother., 2014) References Short, K.R., et.al. (2014). Lancet Infect Dis http://dx.doi.org/10.1016/S1473-3099(13)70286-X Banerejee, I., et.al. (2014). Science. DOI: 10.1126/science.1257037 Wong, W.Y., et.al. (2015). Scientific Reports. DOI:10.1038/srep08672 Belardo, B., et.al. (2014). Antimicrob. Agents Chemother. DOI: 10.1128/AAC.03947-14. New Products   96 Phospho-specific Antibodies Abnova’s phospho-specific antibodies, manufactured under rigorous quality control, are designed to target various phosphorylated proteins with high sensitivity and specificity.

Easter Promotion on Rabbit Antibodies

Let us celebrate Easter! From March 31st to April 14th, 2015, a special 15% discount is offered on 960+ selected Rabbit Antibodies which includes 140 Rabbit Monoclonal Antibodies and 827 Rabbit Polyclonal Antibodies. On top of the special discount, you will also have a chance to receive our Abnova mascot, so hurry-up and join us on the Easter Egg Hunt! Why choose Rabbit Mab over Mouse Mab:   Rabbit Mab Mouse Mab Immune Tolerance No Yes Antibody Diversity Broad Narrow Affinity Picomolar Nanomolar Specificity High Moderate With extensive experience in generating high quality antisera to a wide variety of antigens, Abnova is able to generate high standard rabbit antibodies under strigent quality control to provide more specificity, sensitivity and consistency in detection. High Quality Imaging for both Rabbit Mab and Pab: H2AFX (phospho S139) polycolonal antibody (Cat # PAB0003) Immunofluorescent staining of MCF-7 that has been treated with 10 uM Camptothecin for 30 min using H2AFX (phospho S139) polyclonal antibody. PRKCB monoclonal antibody, clone A10-F (Cat # MAB1630) Immunofluorescent staining of HEK293 cells with PRKCB monoclonal antibody at 1:150 dilution. more selected Rabbit Antibodies on Sale with IF Application >> CCR7 polyclonal antibody (Cat # PAB14776) Immunohistochemistry staining of formalin/PFA-fixed paraffin-embedded human small intestine with CCR7 polyclonal antibody. ERBB3 monoclonal antibody, clone SP71 (Cat # MAB10027) Immunohistochemical staining of paraffin-embedded human small intestine sections with ERBB3 monoclonal antibody, clone SP71. more selected Rabbit Antibodies on Sale with IHC Application >> The Easter Egg Hunt Promotion details: *Discount Antibodies are labeled with a Easter Egg Tag. Direct purchase only, Order via Fax, Phone or E-mail please provide the promotion code: #HappyEaster#. Online orders do not require promotion code, final discount price will be listed in the cart summary. Purchase any selected rabbit antibodies during the Easter promotion, you will have a chance to get a surprise from us! Limited quantities only. Please visit our website and get geared up! Terms and Conditions* Orders must be placed on or before April. 14, 2015 to qualify. This offer cannot be combined with any other offers. No price adjustments to prior purchases. Distributors' sales are excluded. Abnova reserves the right to change the terms and conditions of sale without notice at anytime.