Neurodegenerative Disorder

New Discoveries on Neurodegenerative Disorder Strong evidence has emerged to implicate disturbed mitochondrial fusion and fission as central pathological components underpinning a number of neurodegenerative disorders. Imbalances in mitochondrial fusion and fission affects respiratory chain function, mitochondrial quality control (mitophagy), and programmed cell death. The same cellular processes are also implicated in more-common complex neurodegenerative disorders, such as Alzheimer disease and Parkinson disease. This indicates a common pathological thread and a close relationship among mitochondrial structure, function and localization. (Nature Reviews Neurology, 2015) A mitochondrial fission arrest phenotype may cause elongated interconnected organelles, "mitochondria-on-a-string" (MOAS). Findings suggest that MOAS formation may occur at the final stages of fission process and was not associated with altered translocation of activated dynamin related protein 1 (Drp1) to mitochondria but with reduced GTPase activity. The findings on this research brings new attention to the major role of mitochondrial dynamics in regulating neuronal survival. (Scientific Reports, 2016) Mutations in Parkin and PINK1 cause an inherited early-onset form of Parkinson's disease. The two proteins function together in a mitochondrial quality control pathway. Here, a binding switch between phospho-ubiquitin (pUb) and the ubiquitin-like domain (Ubl) of Parkin was found to play a key role in the release of auto-inhibited ubiquitin ligase activity of Parkin. (The EMBO J, 2015) Neurodegenerative Disorders Alzheimer's Disease Abs Huntington's Disease Abs Parkinson's Disease Abs Mitochondria Implicated Neurodegenerative Disorder Pathways (Click to see your products of interest) Eight Product Categories and 1000+ Products 250 Monoclonal Antibodies 397 Polyclonal Antibodies 35 Antibody Pairs 201 Recombinant Proteins 8 ELISA and Assay Kits 189 RNAi 9 FISH Probes 100 Cell Transient Overexpression Lysates Featured Products IGH/BCL2 DY Translocation FISH Probe FT0011 Fluorescent In Situ Hybridization of human cholangiocarcinoma (FFPE) with IGH/BCL2 DY Translocation FISH Probe (Cat # FT0011). IGH (FITC) BCL2 (Texas Red) PARK2 monoclonal antibody (M01), clone 1H4 H00005071-M01 Immunofluorescence of HeLa Cells with PARK2 monoclonal antibody (Cat # H00005071-M01) at 10 ug/mL. BCL2L1 & BAX Protein Protein Interaction Antibody Pair DI0068 Proximity Ligation Assay of HeLa cells with Anti-BCL2L1 rabbit purified polyclonal antibody (1:1200) and anti-BAX mouse monoclonal antibody (1:50). DAPI (blue) References Florence Burté, et.al. (2015). Nature Review Neurology. DOI: 10.1038/nrneurol.2014.228 Liang Zhang, et. al. (2016) Nature Scientific Reports, DOI: 10.1038/srep18725 Véronique Sauvé, et. al. (2015) The EMBO Journal. DOI: 10.15252/embj.201592237 New Products   200 New Monoclonal antibodies for IHC staining to accelerate your work with precision and accuracy

RNAi Regulations

New Discoveries through RNA interference RNA interference has been developed as methods to evaluate IKK-NF-κB pathway in lung cancer through the influence of IKKα or IKKβ inhibition on NF-κB transcriptional activity and the effects of siRNA-mediated IKK inhibition on cancer proliferation. (Methods in Molecular Biology,2015) High-throughput RNAi kinome screen targeting 720 kinases has identified PDK1 as a factor mediating resistance to CDK4/6 inhibition; thus, it can be a potential drug target in combination with CDK4/6 inhibitor LEE011 to treat ER+ breast cancer. (Cancer Research,2015) Through RNA interference screen, genes, such as TACR1, were identified as factors involved in stress response of mammalian cells and further provides new approaches to reduce the mortality caused by renal ischemia. (Cell Death & Differentiation,2015) RNAi Pathways (Click to see your products of interest) 16 Product Categories 15000+ Products   Antibody Validated Chimera Pre-design Chimera Angiogenesis 2 40 Apoptosis 14 470 Binding 65 2671 Cell Ad/ Junc/ Cytoskeleton 10 535 Cell Cycle 30 704 Cytokine N/A 56 Enzyme 52 793 Membrane 40 2778 Metabolism 13 333 Neurobiology 3 151 Plasma/ Serum 25 860 Signal Transduction 13 623 Stem Cell 10 239 Transcriptions 45 957 Ubiquitin 6 441 Others 45 3200 Featured Products EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 H00027161-M01 Immunohistochemistry staining of formalin fixed paraffin embedded human stomach with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 at 3 ug/mL. NBR1 Validated Chimera RNAi H00004077-R01V Western blot analysis of NBR1 over-expressed 293 cell line, cotransfected with NBR1 Validated Chimera RNAi. Lane 1. Non-transfected control Lane 2. Transfected with NBR1 validated chimera RNAi Blot was probed with NBR1 monoclonal antibody (M05), clone 5C3 (Cat # H00004077-M05 ). GAPDH was used as loading control. TARDBP Validated Chimera RNAi H00023435-R01V Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi. Lane 1. Non-transfected control Lane 2. Transfected with TARDBP validated chimera RNAi Blot was probed with TARDBP monoclonal antibody (M01), clone 2E2-D3 (Cat # H00023435-M01 ). GAPDH was used as loading control. References Bassères, D.S., et.al. (2015). Methods in Molecular Biology. DOI:10.1007/978-1-4939-2422-6_27 Jansen, V.M., et.al. (2015). Cancer Research. DOI:10.1158/1538-7445.AM2015-2844 Zynda, E.R.., et. al. (2015). Cell Death & Differentiation. DOI:10.1038/cdd.2015.128 New Products   High standard IHC antibodies, Rabbit monoclonal antibodies and FISH Probes to fullfill your needs