Bioactive Kinases

New Discoveries on Bioactive Kinases MET receptor tyrosine kinase is regulated by suppresor of cytokine signaling 1 (SOCS1) to attenuate oncogenic MET signaling in hepatocellular carcinoma (HCC) and other cancers. MET inhibitors may be useful in treating HCC patients. (Oncogen,2015) EGFR tyrosine kinase inhibitors triggers innate drug resistance through the inhibition of Akt activity and inactivation of Ets-1 to enhance NSCLC cell survival . (PNAS,2015) Phosphorylation of Ser293 on α-subunit of pyruvate dehydrogenase complex by PDK3 causes meiotic spindle morphology disruption, chromosome dis-alignment and ATP level reduction in oocyte maturation. (Molecular and Cellular Endocrinology,2015) Ten Kinase Categories and 394 Products (Click to see your products of interest) 42 Serine / Threonine Kinase: AGC Group 10 Serine / Threonine Kinase: CK1 Group 38 Serine / Threonine Kinase: STE Group 24 Serine / Threonine Kinase: Other Group 145 Tyrosine Protein Kinase 54 Serine / Threonine Kinase: CAMK Group 26 Serine / Threonine Kinase: CMGC Group 22 Serine / Threonine Kinase: TKL Group 6 Serine / Threonine Kinase: Atypical Group 27 Other Kinase Crosstalk Between Kinases and Apoptotic Proteases in Cancer (Click to see your products of interest) This figure has been adapted and altered from López-Otín, C. & Hunter, T., (Nature Revews Cancer,2015). Featured Products BMPR2 (Human) Recombinant Protein P5499 The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein. After kinase reaction was stopped by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: ALK4 inactive mutant. ATP: 100 uM. IRAK4 (Human) Recombinant Protein P5566 The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: IRAK1 peptide. ATP: 1000 uM RET (M918T) (Human) Recombinant Protein P5631 The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CSK peptide. ATP: 100 uM. References Gui, Y., et. al. (2015). Oncogene. DOI:10.1038/onc.2015.20 Phuchareon, J., et.al. (2015). PNAS. DOI:10.1073/pnas.1510733112 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 López-Otín, C. & Hunter, T. (2015). Nature Reveiws Cancer. DOI:10.1038/nrc2823 New Products   200+ High quality antibodies for immunohistochemistry, immunofluorescence or flow cytometry

Cytoskeleton

New Discoveries on Cytoskeletons Self-organization of actin cytoskeleton, the dynamic of radial fibers-transverse fibers system, takes crucial role in developing the left-right asymmetry in tissues and embryos. (Nature Cell Biology,2015) Apical and basolateral spectrin cytoskeletons, potential mechano-sensors, regulate Hippo signaling pathway in response to human epithelial cell density. (EMBO,2015) Cytoskeleton-associated proteins, CRMP2/DPYSL2, TPM2, TCP1ε and 14.3.3γ, can be potential biomarkers for small-intestinal neuroendocrine tumors invasion. (Molecular and Cellular Endocrinology,2015) Cytoskeleton Compositions (Click to see your products of interest) Proteins Involved in Cytoskeletons (Click to see your products of interest) Actin Polymerization Protein Function/Activity Cofilin Dissociation from (-) end Severin Severing, capping (+) end, control of length of actin filaments; essential for branch formation Gelsolin Severing, capping (+) end, control of length of actin filaments; essential for branch formation CapZ capping protein Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction Tropomodulin Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction ARPC 2/3 Capping (-) end, side binding and nucleation Classes of Intermediate Filaments Protein Function/Activity I. Acidic cytokeratins Mechanical strength II. Basic cytokeratins Mechanical strength III. Vimentin III. Desmin III. GFAP Maintenance of cell shape Structural support for contractile machinery Maintenance of cell shape IV. Neurofilament (NEFL,NEFM,NEFH) Axon strength; Determines axon size V. Nuclear Lamins (Lamin A, Lamin B) Form nuclear scaffold and shape the nucleus VI. Nestin Unknown Modulation of Microtubule Dynamics Protein Function MAP1 Assembles and stabilizes microtubules MAP2 Assembles and cross-links microtubules to one another and intermediate filaments MAP4 Stabilizes microtubules MAPT Assembles and stabilizes and cross-links microtubules RSN/CLIP1 Cross-links microtubules to chromosomes and endosomes Katanin Microtubule severing STMN1/OP18 Binds to tubulin dimers Eight Product Categories and 2100+ Products 654 Monoclonal Antibodies 655 Polyclonal Antibodies 106 Antibody Pairs 310 Recombinant Proteins 34 ELISA Kits 234 RNAi 15 FISH Probe 135 Cell Transient Overexpression Lysates Featured Products TUBA1A monoclonal antibody (M06), clone 2D2 H00007846-M06 Immunofluorescent staining of HeLa cell using TUBA1A monoclonal antibody (Cat #H00007846-M06) at 10ug/ml. ACTA monoclonal antibody (M02), 4A8-2H3 H00000059-M02 Detection limit for recombinant GST tagged ACTA2 using ACTA2 monoclonal antibody (Cat # H00000059-M02) at approximately 1ng/mL as capture antibody. CTNNA1 polyclonal antibody PAB7024 Immunohistochemistry staining of paraffin embedded human prostate using CTNNA1 polyclonal antibody (Cat# PAB7024) at 3.8μg/mL. References Tee, Y.H., et. al (2015). Nature Cell Biology. DOI: 10.1038/ncb3137 Fletcher, G.C., et.al.(2015). EMBO Journal. DOI 10.15252/embj.201489642 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 New Products   42 High quality Metabolism Assay Kits for the detection and quantification of target proteins 15 Cancer research related new products

Hematopoiesis

New Discoveries on Hematopoiesis Hematopoietic stem cell (HSC) is generated from the hemogenic endothelium (HE), where HE and arterial vascular endothelium (VE) was found to represent separate lineages, on the basis of differential expression of CD73 and CD184. (Nature Cell Biology, 2015) HSC regenerative capacity was increased through the inactivation of a histone deacetylase, SIRT7. The interplay of SIRT7 and NRF1 deregulates an unfolded protein response (UPRmt)-mediated metabolic checkpoint which was defined as a reversible contributing factor for HSC aging. (Science, 2015) TET1, the founding member of TET family, was reported as an epigenetic guardian for lymphomagenesis and the TET1 deficiency contributed to predisposed development of B cell malignancy. (Nature Immunology, 2015) Blood Cell Specific Markers (Click to see your products of interest) Cell Type Markers (Human) Markers (Mouse) Hematopoietic Stem Cells Lin-, CD34+, CD38-, FLT3+, THY1+ Lin-, Slamf1 (Cd150)+, Kit+, Casp3 (Sca-1)+ Lin- MS4A1 (CD20), GYPA (CD235a), CD3, NCAM1 (CD56) Ptprc (Cd45ra), Itgam (Cd11b), Cd2, Cd3, Cd4, Cd5, Cd8, Ly6g, Klrb1c, Ly76 Common Lymphoid Progenitor (CLP) MME (CD10)+, CD34+, IL7R (CD127)+ Cd34+, Il7r (Cd127)+ Common Myelold Progenitor (CMP) CD34+, IL3RA (CD123)+, FLT3+ Cd34+, Il3ra (Cd123)+, Casp3 (Sca-1)- Granulocyte/Macrophage Progenitor (GMP) CD34+, CD38+, IL3RA (CD123)+ Cd34+, Cd38+, Il3ra (Cd123)+ Megakaryocyte/Erythroid Progenitor (MEP) CD38+, PTPRC (CD45RA)- Cd38+, Ptprc (Cd45ra)- Megakaryocytes ITGA2B (CD41)+, MT2A (CD42)+, MPL (TPOR)+ Itga2b (Cd41)+, Mt2a (Cd42)+, Mpl (Tpor)+ Monocytes CD14+, CXCR1+, ITGAM (CD11b)+, CD33 (Siglec-3)+ Cd14+, Cxcr1+, Itgam (Cd11b)+, Cd33 (Siglec-3)+ Lymphocytes LY9+, SLAMF7+ Ly9+, Slamf7+ Natural Killer Cells (NK Cells) NCAM1 (CD56)+, CD16+ Ncr1 (Cd335) +, Fcgr3 (CD16)+ B Cells CD19+, MS4A1 (CD20)+ Ptprc (Cd45ra)+, Cd19+, Cd22+ T Cells CD2+, CD3+, CD4+, CD8+ Cd2+, Cd3+, Cd4+, Cd8+ Granulocytes CCR3+, ITGAM (CD11b)+, ANPEP (CD13)+, CD16+, ITGB2 (CD18)+ Ccr3+, Itgam (Cd11b)+, Anpep (Cd13)+, Fcgr3 (Cd16)+, Itgb2 (Cd18)+ Basophils ANPEP (CD13)+, CD164+, IL3RA (CD123)+, LAMP1 (CD107a)+ Anpep+, Cd164+, Il3ra (CD123)+, Lamp1 (Cd107a)+ Neutrophils CD66+, SELL (CD62L)+, MPO+ Ceacam1 (Cd66)+ , Sell+, Mpo+ Eosinophils CD44+, CD69+, EMR1+ Cd44+, Cd69+, Emr1+ Erythrocytes GYPA (CD235a)+, CD59+ Gypa (Cd235a)+, Cd59a+ Dendritic Cells IL3RA (CD123)+, CD80+, CD83+, CD86+, HLA-DRA+ Il3ra (Cd123)+, Cd80+, Cd83+, Cd86+ Macrophages CD14+, CD163+, CD36+, CD68+ Cd14+, Cd163+, Cd36+, Cd68+ Platelets ITGA2B (CD41)+, ITGB3 (CD61)+, PEAR1+ Itga2b (Cd41)+, Itgb3 (Cd61)+, Pear1+ Hematopoiesis (Click to see your products of interest) Eight Product Categories and 500+ Products 224 Monoclonal Antibodies 98 Polyclonal Antibodies 14 Antibody Pairs 109 Recombinant Proteins 69 ELISA Kits and ELISpot Kits 19 RNAi 1 FISH Probe 19 Cell Transient Overexpression Lysates Featured Products IL6 polyclonal antibody PAB27750 Immunohistochemistry staining of paraffin-embedded human lung tissue using IL6 polyclonal antibody (Cat # PAB27750). EPO monoclonal antibody, clone 4F11 MAB10637 Immunofluorescence analysis of GC-7901 cells using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Western blot analysis using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) against cell lysates. Lane 1: HEK293 Lane 2: EPO-hIgGFc transfected HEK293 References Ditadi A, et.al. (2015). Nature Cell Biology. DOI: 10.1038/ncb3161. Mohrin M, et. al. (2015) Science. DOI: 10.1126/science.1258849. Rasmussen KD, et. al. (2015) Nature Immunology. DOI: 10.1038/ni.3176 New Products   36 Antibodies specific for CD markers in flow cytometry to identify your targets 15 High quality ELISA Kits for the detection and quantification of specific target proteins

Kiss of Death - Ubiquitylation

New Discoveries on Ubiquitylation Elongation of ubiquitin chain, regardless of the linkage, can form polyubiquitin fibrils to initiate autophagy pathway. Incapacity of autophagy can cause cytotoxic accumulation of ubiquitin-positive aggregates leading to neurodegenerative diseases. (Nature communication,2015) Highly expressed in human breast and lung cancers, a new deubiquitinating enzyme, USP36, was identified to regulate c-Myc oncoprotein stability in nucleolus. (PNAS,2015) Itch, an E3 HECT ubiquitin ligase, inhibits MAPK p38α activation through ubiquitylation can be exploited therapeutically to prevent chronic skin inflammation. (Science,2015) Tumor Related Misregulated Proteins in Ubiquitin Modification (Click to see your products of interest) Tumor Type Deregulated Protein Substrate Modification Non-Small-Cell Lung Cancer MDM2 (39) p53/TP53 (151) Polyubiquitylation HAUSP/USP7 (8) MDM2(39) p53/TP53 (151) De-ubiquitylation Renal Cell Carcinoma VHL (16) HIF/HIF1/HIF1α (23) De-ubiquitylation Colorectal Cancer APC (69) Cyclin B (31) Securin Polyubiquitylation Lymphoma KITLG/SCF (25) CDKN1B/p27 (51) Polyubiquitylation Fanconi Anaemia-Related Cancer ATAD3A/FANCL (9) FANCD2 (9) Monoubiquitylation MALT Lymphomas IAP2 BCL10 (15) Polyubiquitylation Lymphoma, AML, Gastric Carcinoma CBL (33) RTKs Multiple Monoubiquitylation Cervical Cancer UBE3A/E6-AP (11) p53/TP53 (151) Polyubiquitylation Breast Cancer BRCA1 (75) Unknown Unknown CBL (33) EGFR (117) De-ubiquitylation Ovarian Cancer F-box protein Cyclin E/CCNE1 (43) De-ubiquitylation ( ) Number of Products Provided by Abnova Tumor Related Misregulated Proteins in Ubiquitin Modification (Click to see your products of interest) Eight Product Categories and 1485 Products 296 Monoclonal Antibodies 529 Polyclonal Antibodies 153 Antibody Pairs 183 Recombinant Proteins 44 Assay Kits and ELISA Kits 180 RNAi 17 FISH Probes 80 Cell Transient Overexpression Lysates Newly Cited Products FBXW7 monoclonal antibody (M02), clone 3D1 H00055294-M02 Yumimoto, K., et al. determined expression of FBXW7, the F-box protein of SCF ubiquitin ligase, can suppress cancer metastasis in either non-cell-autonomous or cell-autonomous manner. Abnova’s FBXW7 monoclonal antibody was used in this study to perform immunocytochemical staining on breast tissues. (JCI, 2015) Immunohistochemistry staining of formalin-fixed paraffin-embedded human lung using FBXW7 monoclonal antibody (3ug/ml). Western Blot detection against FBXW7 partial recombinant protein with GST tag (37.73KDa). CCNE1/CEN19p FISH Probe FG0013 Abnova’s FG0013 CCNE1/CEN19p FISH Probe was recently used by Noske, A., et.al. to evaluate CCNE 1 copy number in the investigation of Cyclin E and URI gene amplification as well as protein expression in different epithelial ovarian cancer subtypes. (Experiment and Molecular Pathology, 2015) Normal human lymphocyte stained with the CCNE1/CEN19p dual color FISH probe at interphase nucleus. Human ovary cancer (FFPE) stained with CCNE1/CEN19p FISH Probe. Human ovary cancer showed no CCNE1 gene amplification. References Morimoto, D., et.al. (2015). Nature communications. DOI: 10.1038/ncomms7116 Sun, X.-X., et.al. (2015). PNAS. DOI: 10.1073/pnas.1411713112 Theivanthiran, B., et.al. (2015). Science. DOI: 10.1126/scisignal.2005903 Yumimoto, K., et.al. (2015) JCI. DOI: 10.172/JCI78782 Noske, A., et.al. (2015). Experiment and Molecular Pathology. DOI: 10.1016/j.yexmp.2014.12.004 New Products   3 FISH Probes for Detecting Gene Amplification in Cancer Cells 49 Pathology Antibodies for High Quality Immunohistochemistry Staining 102 Antibody Pairs for ELISA - Sensitive Detection of Target Proteins