TGFβ Signaling Pathway

New Discoveries on TGFβ Signaling Pathway ITGBL1, as an upstream effector of TGFβ signaling pathway, is a key contributor to osteomimetic breast cancer. (Cancer Research,2015) Through the activation of TGFβ signaling pathway, HOXB7 promotes malignant tumor progression in cell-autonomous and non-cell-autonomous manners. (Cancer Research,2015) Colon carcinogenesis is strongly associated with genetic mutations in TGFβ signaling pathway of inflamed and regenerating intestinal mucosa. (Cancer Research,2015) TGFβ / SMAD Signaling Pathway (Click to see your products of interest)

Eight Product Categories and 900+ Products
221 Monoclonal Antibodies 301 Polyclonal Antibodies 141 Antibody Pairs 111 Recombinant Proteins	20 ELISA and Assay Kits 76 RNAi 10 FISH Probes 22 Cell Transient Overexpression Lysates

Featured Products
MAP2K4 purified MaxPab mouse polyclonal antibody (B01P) H00006416-B01P
	Immunofluorescent staining of HeLa cell using MAP2K4 purified MaxPab mouse polyclonal antibody (Cat# H00006416-B01P) at 10 ug/mL.
LIMK1 monoclonal antibody (M01), clone 1A8 H00003984-M01
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human stomach with anti-LIMK1 monoclonal antibody, clone 1A8 (Cat# H00003984-M01) at 3 ug/mL.
SHC1 monoclonal antibody (M01), clone 3F4 H00006464-M01
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human stomach with anti-SHC1 monoclonal antibody, clone 3F4 (Cat# H00006464-M01) at 1 ug/mL.

References

Li, X.-Q., et.al. (2015). Cancer Research. DOI:10.1158/0008-5472.CAN-15-0240 Liu, S., et.al. (2015). Cancer Research. 10.1158/0008-5472.CAN-14-3100 Oshima H., et. al. (2015). Cancer Research. DOI:10.1158/0008-5472.CAN-14-2036

New Products

300+ High quality antibodies and kits designed for Enzyme-Linked ImmunoSorbent Assay (ELISA)

Diabetes Research

New Discoveries on Diabetes Leptin was found with the capability of suppressing diabetic hyperglucagonemia through two physiologic pathways, prevention of potentially toxic lipids accumulation and unrecognization of paracrine interactions between α and β cells. (Cell Metabolism, 2015) As β cell failure is one of the major defects in type 2 diabetes mellitus, locally produced apoCIII was identified as an important diabetogenic factor involved in impairment of β cell function. The evidence may also suggest that apoCIII is potentially a novel target for the treatment of type 2 diabetes mellitus. (PNAS, 2015) Neutrophils isolated from type 1 and type 2 diabetic humans and mice were found to produce extracellular traps (NETs, process termed NETosis), which impaired wound healing particularly in diabetes. The evidence supported that inhibiting NETosis or cleaving NETs might improve wound healing and reduce NET-driven chronic inflammation in diabetes. (Nature Medicine, 2015) Type I Diabetes Pathway (Click to see your products of interest) Human/Mouse

Kanehisa Laboratories. (2010, May 23). This figure has been adapted and altered from KEGG.

Type II Diabetes Pathway (Click to see your products of interest) Human/Mouse

Kanehisa Laboratories. (2015, Feb 13). This figure has been adapted and altered from KEGG.

Eight Product Categories and 1800+ Products
618 Monoclonal Antibodies 484 Polyclonal Antibodies 191 Antibody Pairs 224 Recombinant Proteins	92 Assay Kits and ELISA Kits 138 RNAi 14 FISH Probes 81 Cell Transient Overexpression Lysates

Featured Diabetes Related Products
PTPRN2 purified MaxPab mouse polyclonal antibody (B01P) Cat # H00005799-B01P
	Immunohistochemistry staining of formalin-fixed paraffin-embedded human pancreas using PTPRN2 purified MaxPab mouse polyclonal antibody (B01P) (Cat # H00005799-B01P) at 3 ug/mL.
MAPK3 monoclonal antibody (M01), clone 3C9 Cat # H00005595-M01

Immunohistochemistry staining of formalin-fixed paraffin-embedded human placenta using MAPK3 monoclonal antibody (M01), clone 3C9 (Cat # H00005595-M01) at 3 ug/mL.

HLA-A purified MaxPab mouse polyclonal antibody (B01P)
Cat # H00003105-B01P

FACS analysis of negative control 293 cells (Black) and HLA-A expressing 293 cells (Green) using HLA-A purified MaxPab mouse polyclonal antibody (Cat # H00003105-B01P).

PRKCD monoclonal antibody (M06), clone 2E12
Cat # H00005580-M06

Immunofluorescence staining of A-431 cells using PRKCD monoclonal antibody (M06), clone 2E12 (Cat # H00005580-M06) at 10 ug/mL.

IKBKB & CREBBP Protein Protein Interaction Antibody Pair
Cat # DI0278

Proximity Ligation Assay of protein-protein interactions between IKBKB and CREBBP.
HeLa cells were stained with anti-IKBKB rabbit purified polyclonal antibody 1:1200 and anti-CREBBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex.

References

Unger, R., et.al. (2015). Cell Metabolism. DOI: 10.1016/j.cmet.2014.10.011 Avall, K., et.al. (2015). PNAS. DOI: 10.1073/pnas.1423849112 Wang, S.-L., et.al. (2015). Nature Medicine. DOI: 10.1038/nm.3887

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140 Phospho Specific Antibodies and Kits Specified for the Study of Phosphorylation 55+ New Antibodies with High Qulity for Flow Cytometry Analysis

New IHC Antibodies

Abnova offers 200+ new antibodies with high specificity and sensitivity for a great variety of applications including enzyme-linked immunosorbent assay (ELISA), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC) and flow cytometry. Among the new antibodies, we gladly introduce 193 antibodies for immunohistochemistry (IHC) staining with high consistancy to accelerate your research. Please check the following information of new antibodies: New IHC Antibodies: Angiogenesis Apoptosis Binding (Nucleic Acid/Others) Cell Ad/Junc/Cytoskel Cell Cycle Cytokine Enzyme (Kinase/Others) Membrane (Transporter/Receptor/Others) Metabolism Neurobiology Plasma/Serum Signal Transduction Stem Cell Transcriptions Ubiquitin Others Featured IHC Antibodies: Catalog # Product Name PAB29557 MAP1LC3A (Cleaved) polyclonal antibody PAB29558 MAP1LC3A polyclonal antibody MAB12245 FTL monoclonal antibody, clone 6E10E4 MAB12260 CD68 monoclonal antibody, clone 3F7D3 MAB12264 CDKN2A monoclonal antibody, clone 1D7D2A1 Other New Antibodies: Apoptosis Binding (Nucleic Acid/Others) Cell Ad/Junc/Cytoskel Cell Cycle Enzyme (Kinase/Others) Membrane (Transporter/Receptor/Others) Metabolism Ubiquitin

Bioactive Kinases

New Discoveries on Bioactive Kinases MET receptor tyrosine kinase is regulated by suppresor of cytokine signaling 1 (SOCS1) to attenuate oncogenic MET signaling in hepatocellular carcinoma (HCC) and other cancers. MET inhibitors may be useful in treating HCC patients. (Oncogen,2015) EGFR tyrosine kinase inhibitors triggers innate drug resistance through the inhibition of Akt activity and inactivation of Ets-1 to enhance NSCLC cell survival . (PNAS,2015) Phosphorylation of Ser293 on α-subunit of pyruvate dehydrogenase complex by PDK3 causes meiotic spindle morphology disruption, chromosome dis-alignment and ATP level reduction in oocyte maturation. (Molecular and Cellular Endocrinology,2015) Ten Kinase Categories and 394 Products (Click to see your products of interest) 42 Serine / Threonine Kinase: AGC Group 10 Serine / Threonine Kinase: CK1 Group 38 Serine / Threonine Kinase: STE Group 24 Serine / Threonine Kinase: Other Group 145 Tyrosine Protein Kinase 54 Serine / Threonine Kinase: CAMK Group 26 Serine / Threonine Kinase: CMGC Group 22 Serine / Threonine Kinase: TKL Group 6 Serine / Threonine Kinase: Atypical Group 27 Other Kinase Crosstalk Between Kinases and Apoptotic Proteases in Cancer (Click to see your products of interest) This figure has been adapted and altered from López-Otín, C. & Hunter, T., (Nature Revews Cancer,2015). Featured Products BMPR2 (Human) Recombinant Protein P5499 The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein. After kinase reaction was stopped by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: ALK4 inactive mutant. ATP: 100 uM. IRAK4 (Human) Recombinant Protein P5566 The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: IRAK1 peptide. ATP: 1000 uM RET (M918T) (Human) Recombinant Protein P5631 The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: CSK peptide. ATP: 100 uM. References Gui, Y., et. al. (2015). Oncogene. DOI:10.1038/onc.2015.20 Phuchareon, J., et.al. (2015). PNAS. DOI:10.1073/pnas.1510733112 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 López-Otín, C. & Hunter, T. (2015). Nature Reveiws Cancer. DOI:10.1038/nrc2823 New Products   200+ High quality antibodies for immunohistochemistry, immunofluorescence or flow cytometry

Cytoskeleton

New Discoveries on Cytoskeletons Self-organization of actin cytoskeleton, the dynamic of radial fibers-transverse fibers system, takes crucial role in developing the left-right asymmetry in tissues and embryos. (Nature Cell Biology,2015) Apical and basolateral spectrin cytoskeletons, potential mechano-sensors, regulate Hippo signaling pathway in response to human epithelial cell density. (EMBO,2015) Cytoskeleton-associated proteins, CRMP2/DPYSL2, TPM2, TCP1ε and 14.3.3γ, can be potential biomarkers for small-intestinal neuroendocrine tumors invasion. (Molecular and Cellular Endocrinology,2015) Cytoskeleton Compositions (Click to see your products of interest) Proteins Involved in Cytoskeletons (Click to see your products of interest) Actin Polymerization Protein Function/Activity Cofilin Dissociation from (-) end Severin Severing, capping (+) end, control of length of actin filaments; essential for branch formation Gelsolin Severing, capping (+) end, control of length of actin filaments; essential for branch formation CapZ capping protein Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction Tropomodulin Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction ARPC 2/3 Capping (-) end, side binding and nucleation Classes of Intermediate Filaments Protein Function/Activity I. Acidic cytokeratins Mechanical strength II. Basic cytokeratins Mechanical strength III. Vimentin III. Desmin III. GFAP Maintenance of cell shape Structural support for contractile machinery Maintenance of cell shape IV. Neurofilament (NEFL,NEFM,NEFH) Axon strength; Determines axon size V. Nuclear Lamins (Lamin A, Lamin B) Form nuclear scaffold and shape the nucleus VI. Nestin Unknown Modulation of Microtubule Dynamics Protein Function MAP1 Assembles and stabilizes microtubules MAP2 Assembles and cross-links microtubules to one another and intermediate filaments MAP4 Stabilizes microtubules MAPT Assembles and stabilizes and cross-links microtubules RSN/CLIP1 Cross-links microtubules to chromosomes and endosomes Katanin Microtubule severing STMN1/OP18 Binds to tubulin dimers Eight Product Categories and 2100+ Products 654 Monoclonal Antibodies 655 Polyclonal Antibodies 106 Antibody Pairs 310 Recombinant Proteins 34 ELISA Kits 234 RNAi 15 FISH Probe 135 Cell Transient Overexpression Lysates Featured Products TUBA1A monoclonal antibody (M06), clone 2D2 H00007846-M06 Immunofluorescent staining of HeLa cell using TUBA1A monoclonal antibody (Cat #H00007846-M06) at 10ug/ml. ACTA monoclonal antibody (M02), 4A8-2H3 H00000059-M02 Detection limit for recombinant GST tagged ACTA2 using ACTA2 monoclonal antibody (Cat # H00000059-M02) at approximately 1ng/mL as capture antibody. CTNNA1 polyclonal antibody PAB7024 Immunohistochemistry staining of paraffin embedded human prostate using CTNNA1 polyclonal antibody (Cat# PAB7024) at 3.8μg/mL. References Tee, Y.H., et. al (2015). Nature Cell Biology. DOI: 10.1038/ncb3137 Fletcher, G.C., et.al.(2015). EMBO Journal. DOI 10.15252/embj.201489642 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 New Products   42 High quality Metabolism Assay Kits for the detection and quantification of target proteins 15 Cancer research related new products