Cytoskeleton

New Discoveries on Cytoskeletons Self-organization of actin cytoskeleton, the dynamic of radial fibers-transverse fibers system, takes crucial role in developing the left-right asymmetry in tissues and embryos. (Nature Cell Biology,2015) Apical and basolateral spectrin cytoskeletons, potential mechano-sensors, regulate Hippo signaling pathway in response to human epithelial cell density. (EMBO,2015) Cytoskeleton-associated proteins, CRMP2/DPYSL2, TPM2, TCP1ε and 14.3.3γ, can be potential biomarkers for small-intestinal neuroendocrine tumors invasion. (Molecular and Cellular Endocrinology,2015) Cytoskeleton Compositions (Click to see your products of interest) Proteins Involved in Cytoskeletons (Click to see your products of interest) Actin Polymerization Protein Function/Activity Cofilin Dissociation from (-) end Severin Severing, capping (+) end, control of length of actin filaments; essential for branch formation Gelsolin Severing, capping (+) end, control of length of actin filaments; essential for branch formation CapZ capping protein Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction Tropomodulin Capping (+) end, stabilize F-actin prevent disassembly during muscle contraction ARPC 2/3 Capping (-) end, side binding and nucleation Classes of Intermediate Filaments Protein Function/Activity I. Acidic cytokeratins Mechanical strength II. Basic cytokeratins Mechanical strength III. Vimentin III. Desmin III. GFAP Maintenance of cell shape Structural support for contractile machinery Maintenance of cell shape IV. Neurofilament (NEFL,NEFM,NEFH) Axon strength; Determines axon size V. Nuclear Lamins (Lamin A, Lamin B) Form nuclear scaffold and shape the nucleus VI. Nestin Unknown Modulation of Microtubule Dynamics Protein Function MAP1 Assembles and stabilizes microtubules MAP2 Assembles and cross-links microtubules to one another and intermediate filaments MAP4 Stabilizes microtubules MAPT Assembles and stabilizes and cross-links microtubules RSN/CLIP1 Cross-links microtubules to chromosomes and endosomes Katanin Microtubule severing STMN1/OP18 Binds to tubulin dimers Eight Product Categories and 2100+ Products 654 Monoclonal Antibodies 655 Polyclonal Antibodies 106 Antibody Pairs 310 Recombinant Proteins 34 ELISA Kits 234 RNAi 15 FISH Probe 135 Cell Transient Overexpression Lysates Featured Products TUBA1A monoclonal antibody (M06), clone 2D2 H00007846-M06 Immunofluorescent staining of HeLa cell using TUBA1A monoclonal antibody (Cat #H00007846-M06) at 10ug/ml. ACTA monoclonal antibody (M02), 4A8-2H3 H00000059-M02 Detection limit for recombinant GST tagged ACTA2 using ACTA2 monoclonal antibody (Cat # H00000059-M02) at approximately 1ng/mL as capture antibody. CTNNA1 polyclonal antibody PAB7024 Immunohistochemistry staining of paraffin embedded human prostate using CTNNA1 polyclonal antibody (Cat# PAB7024) at 3.8μg/mL. References Tee, Y.H., et. al (2015). Nature Cell Biology. DOI: 10.1038/ncb3137 Fletcher, G.C., et.al.(2015). EMBO Journal. DOI 10.15252/embj.201489642 Couderc, C., et.al. (2015). Molecular and Cellular Endocrinology. DOI:10.1016/j.mce.2014.09.006 New Products   42 High quality Metabolism Assay Kits for the detection and quantification of target proteins 15 Cancer research related new products

Hematopoiesis

New Discoveries on Hematopoiesis Hematopoietic stem cell (HSC) is generated from the hemogenic endothelium (HE), where HE and arterial vascular endothelium (VE) was found to represent separate lineages, on the basis of differential expression of CD73 and CD184. (Nature Cell Biology, 2015) HSC regenerative capacity was increased through the inactivation of a histone deacetylase, SIRT7. The interplay of SIRT7 and NRF1 deregulates an unfolded protein response (UPRmt)-mediated metabolic checkpoint which was defined as a reversible contributing factor for HSC aging. (Science, 2015) TET1, the founding member of TET family, was reported as an epigenetic guardian for lymphomagenesis and the TET1 deficiency contributed to predisposed development of B cell malignancy. (Nature Immunology, 2015) Blood Cell Specific Markers (Click to see your products of interest) Cell Type Markers (Human) Markers (Mouse) Hematopoietic Stem Cells Lin-, CD34+, CD38-, FLT3+, THY1+ Lin-, Slamf1 (Cd150)+, Kit+, Casp3 (Sca-1)+ Lin- MS4A1 (CD20), GYPA (CD235a), CD3, NCAM1 (CD56) Ptprc (Cd45ra), Itgam (Cd11b), Cd2, Cd3, Cd4, Cd5, Cd8, Ly6g, Klrb1c, Ly76 Common Lymphoid Progenitor (CLP) MME (CD10)+, CD34+, IL7R (CD127)+ Cd34+, Il7r (Cd127)+ Common Myelold Progenitor (CMP) CD34+, IL3RA (CD123)+, FLT3+ Cd34+, Il3ra (Cd123)+, Casp3 (Sca-1)- Granulocyte/Macrophage Progenitor (GMP) CD34+, CD38+, IL3RA (CD123)+ Cd34+, Cd38+, Il3ra (Cd123)+ Megakaryocyte/Erythroid Progenitor (MEP) CD38+, PTPRC (CD45RA)- Cd38+, Ptprc (Cd45ra)- Megakaryocytes ITGA2B (CD41)+, MT2A (CD42)+, MPL (TPOR)+ Itga2b (Cd41)+, Mt2a (Cd42)+, Mpl (Tpor)+ Monocytes CD14+, CXCR1+, ITGAM (CD11b)+, CD33 (Siglec-3)+ Cd14+, Cxcr1+, Itgam (Cd11b)+, Cd33 (Siglec-3)+ Lymphocytes LY9+, SLAMF7+ Ly9+, Slamf7+ Natural Killer Cells (NK Cells) NCAM1 (CD56)+, CD16+ Ncr1 (Cd335) +, Fcgr3 (CD16)+ B Cells CD19+, MS4A1 (CD20)+ Ptprc (Cd45ra)+, Cd19+, Cd22+ T Cells CD2+, CD3+, CD4+, CD8+ Cd2+, Cd3+, Cd4+, Cd8+ Granulocytes CCR3+, ITGAM (CD11b)+, ANPEP (CD13)+, CD16+, ITGB2 (CD18)+ Ccr3+, Itgam (Cd11b)+, Anpep (Cd13)+, Fcgr3 (Cd16)+, Itgb2 (Cd18)+ Basophils ANPEP (CD13)+, CD164+, IL3RA (CD123)+, LAMP1 (CD107a)+ Anpep+, Cd164+, Il3ra (CD123)+, Lamp1 (Cd107a)+ Neutrophils CD66+, SELL (CD62L)+, MPO+ Ceacam1 (Cd66)+ , Sell+, Mpo+ Eosinophils CD44+, CD69+, EMR1+ Cd44+, Cd69+, Emr1+ Erythrocytes GYPA (CD235a)+, CD59+ Gypa (Cd235a)+, Cd59a+ Dendritic Cells IL3RA (CD123)+, CD80+, CD83+, CD86+, HLA-DRA+ Il3ra (Cd123)+, Cd80+, Cd83+, Cd86+ Macrophages CD14+, CD163+, CD36+, CD68+ Cd14+, Cd163+, Cd36+, Cd68+ Platelets ITGA2B (CD41)+, ITGB3 (CD61)+, PEAR1+ Itga2b (Cd41)+, Itgb3 (Cd61)+, Pear1+ Hematopoiesis (Click to see your products of interest) Eight Product Categories and 500+ Products 224 Monoclonal Antibodies 98 Polyclonal Antibodies 14 Antibody Pairs 109 Recombinant Proteins 69 ELISA Kits and ELISpot Kits 19 RNAi 1 FISH Probe 19 Cell Transient Overexpression Lysates Featured Products IL6 polyclonal antibody PAB27750 Immunohistochemistry staining of paraffin-embedded human lung tissue using IL6 polyclonal antibody (Cat # PAB27750). EPO monoclonal antibody, clone 4F11 MAB10637 Immunofluorescence analysis of GC-7901 cells using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Western blot analysis using EPO monoclonal antibody, clone 4F11 (Cat # MAB10637) against cell lysates. Lane 1: HEK293 Lane 2: EPO-hIgGFc transfected HEK293 References Ditadi A, et.al. (2015). Nature Cell Biology. DOI: 10.1038/ncb3161. Mohrin M, et. al. (2015) Science. DOI: 10.1126/science.1258849. Rasmussen KD, et. al. (2015) Nature Immunology. DOI: 10.1038/ni.3176 New Products   36 Antibodies specific for CD markers in flow cytometry to identify your targets 15 High quality ELISA Kits for the detection and quantification of specific target proteins